Journal: Journal of Cellular and Molecular Medicine
Article Title: Hypotonic Swelling Method for the Isolation of Pure Mitochondria From Primary Human Skeletal Myoblasts for Proteomic Studies
doi: 10.1111/jcmm.70370
Figure Lengend Snippet: Sample abundance graph of mitochondrial proteins detected by LC–MS/MS analysis of mitochondrial fractions isolated from primary skeletal myoblasts by the hypotonic swelling method. Each sample was run three times by MS. The median values are represented by a horizontal line within each box, while points positioned outside the boxes are identified as outliers. (Control 1: 8063; Control 2: NH11‐1462A).
Article Snippet: Primary human skeletal myoblasts were proliferated in Skeletal Muscle Cell Growth Medium (PromoCell) supplemented with Supplement mix (PromoCell), and 2 mM L‐glutamine in an incubator at 37°C with 5% CO 2 .
Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation, Control